Nature Cell Biology contents: March 2014 Volume 16 Number 3, pp 201 - 293

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Advertisement BD high quality reagents at everyday low prices  Your institutional buying agreement with BD Biosciences allows you to use BD high quality research reagents in your research without spending your time researching promo codes or shopping for discounts. Buy with confidence, because lower prices are already in your basket.  bdbiosciences.com/go/myprice TABLE OF CONTENTS March 2014 Volume 16, Issue 3 Review News and Views Research Highlights Articles Resource Advertisement   Precise Cell Counts in 30 Seconds The Scepter™ 2.0 Handheld Automated Cell Counter enables convenient, accurate cell counting at the bench or cell culture hood. Now you can rapidly determine cell health, count subpopulations in Peripheral Blood Mononuclear Cells (PBMC) preps, and much more — all with the ease and precision of the portable Scepter™ cell counter. Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement The New York Stem Cell Foundation — Robertson Investigator Awards in Neuroscience  The New York Stem Cell Foundation is accepting proposals for high-risk, high-reward projects exploring fundamental areas of developmental, cellular, cognitive, and behavioral neuroscience. The awards provide $1.5M USD over 5 years. Application deadline: March 17, 2014. View the full RFA at www.nyscf.org/neuro   Advertisement nature.com webcasts Macmillan Science Communication presents a custom webcast: Biomarkers and immunoassays: Enhancing your research with multiplex cytokine profiling. Thursday 20 March at 8am PDT / 11am EDT / 3pm GMT / 4pm CET Register for the free webcast followed by a live Q & A session  Sponsored by:      Review Top Impact of genomic damage and ageing on stem cell function   pp201 - 207 Axel Behrens, Jan M. van Deursen, K. Lenhard Rudolph and Björn Schumacher doi:10.1038/ncb2928 In the second Review in our Genomic Instability series, Rudolph and colleagues discuss how the genomic damage that accumulates during ageing affects stem cell function through both cell-intrinsic and cell-extrinsic mechanisms. News and Views Top Rho-directed forces in collective migration   pp208 - 210 Peter Friedl, Katarina Wolf and Mirjam M. Zegers doi:10.1038/ncb2923 Collective cell migration depends on multicellular mechanocoupling between leader and follower cells to coordinate traction force and position change. Co-registration of Rho GTPase activity and forces in migrating epithelial cell sheets now shows how RhoA controls leader-follower cell hierarchy, multicellular cytoskeletal contractility and mechanocoupling, to prevent ectopic leading edges and to move the cell sheet forward. See also: Article by Reffay et al. SIK2 regulates insulin secretion   pp210 - 212 Bengt-Frederik Belgardt and Markus Stoffel doi:10.1038/ncb2925 Correct regulation of insulin secretion by the pancreas is crucial for organismal function and survival. The AMPK-related kinase SIK2 (salt-inducible kinase 2) is now shown to be stabilized in pancreatic β-cells following glucose stimulation, leading to improved systemic glucose homeostasis by regulating cellular calcium flux and insulin secretion. See also: Article by Sakamaki et al. A microRNA-operated switch of asymmetric-to-symmetric cancer stem cell divisions   pp212 - 214 Robin G. Lerner and Claudia Petritsch doi:10.1038/ncb2924 Defective asymmetric cell divisions of stem and progenitor cells are associated with tumorigenesis by a largely unknown mechanism. A signalling axis involving Snail, microRNA-146a and Numb is now shown to regulate the switch between symmetric and asymmetric cell division in colorectal cancer stem cells. See also: Article by Hwang et al. Cell Biology JOBS of the week Postdoctoral Fellow in Stem Cell Biology McEwen Centre for Regenerative Medicine/University Health Network Postdoctoral Position in Hematopoietic Stem Cell Biology St. Jude Children's Research Hospital (SJCRH) Director of Cell Biology (JBEI) Lawrence Berkeley National Laboratory (LBNL) Postdoc In Cell Biology Of Microtubules Vanderbilt University Medical Center More Science jobs from Cell Biology EVENT At the interface of Structural, Chemical, Computational and Cell Biology 17.06.14 London, UK More science events from Research Highlights Top Opposing enzymes cooperate in ERAD | Myosin forces in haematopoiesis | A scaffold for phagophore membranes | Endothelial cells drive epithelial repair in lung Advertisement Bio-Rad introduces the V3 Western Workflow™, providing increased speed and confidence and improving quantitation over compared to your traditional western blotting protocol. Learn how V3 can to improve your blotting results here.    Articles Top Interplay of RhoA and mechanical forces in collective cell migration driven by leader cells   pp217 - 223 M. Reffay, M. C. Parrini, O. Cochet-Escartin, B. Ladoux, A. Buguin et al. doi:10.1038/ncb2917 Silberzan and colleagues demonstrate that local RhoA activity and mechanical forces control the formation of 'migration fingers', cell protrusions involved in the leader-cell-driven collective migration of epithelial cell monolayers. See also: News and Views by Friedl et al. Mechanical control of the sense of touch by β-spectrin   pp224 - 233 Michael Krieg, Alexander R. Dunn and Miriam B. Goodman doi:10.1038/ncb2915 How sensory neurons integrate mechanical signals during touch sensation has remained unclear. Using a combination of laser axotomy and FRET imaging to measure force across single cells and molecules, Goodman and colleagues show that the neuronal spectrin cytoskeleton transduces touch sensation in C. elegans. Role of the SIK2–p35–PJA2 complex in pancreatic β-cell functional compensation   pp234 - 244 Jun-Ichi Sakamaki, Accalia Fu, Courtney Reeks, Stephen Baird, Chantal Depatie et al. doi:10.1038/ncb2919 Screaton and colleagues delineate a pathway involving the AMPK-related Sik2 kinase using mouse models. They show that Sik2-mediated phosphorylation and subsequent degradation of CDK5R1 (p35) by PJA2 is needed for glucose-induced insulin secretion and β-cell functional compensation in models of hyperglycemia and obesity. See also: News and Views by Belgardt & Stoffel α-catenin acts as a tumour suppressor in E-cadherin-negative basal-like breast cancer by inhibiting NF-κB signalling   pp245 - 254 Hai-long Piao, Yuan Yuan, Min Wang, Yutong Sun, Han Liang et al. doi:10.1038/ncb2909 Ma and colleagues report that in E-cadherin-deficient basal-like breast cancer cells, α-catenin acts as a tumour suppressor by interacting with and stabilizing IκBα, leading to inhibition of NF-κB signalling. The chromatin regulator Brg1 suppresses formation of intraductal papillary mucinous neoplasm and pancreatic ductal adenocarcinoma   pp255 - 267 Guido von Figura, Akihisa Fukuda, Nilotpal Roy, Muluye E. Liku, John P. Morris IV et al. doi:10.1038/ncb2916 Hebrok and colleagues use mouse models to demonstrate that loss of the chromatin modifier Brg1 cooperates with oncogenic KRas to form lesions resembling intraductal papillary mucinous neoplasia that progress to pancreatic adenocarcinoma. MicroRNA-146a directs the symmetric division of Snail-dominant colorectal cancer stem cells   pp268 - 280 Wei-Lun Hwang, Jeng-Kae Jiang, Shung-Haur Yang, Tse-Shun Huang, Hsin-Yi Lan et al. doi:10.1038/ncb2910 Wang, Yang and colleagues delineate a Snail–β-catenin-miR-146a signalling axis that directs a switch from asymmetric to symmetric cell division, resulting in colorectal cancer stem cell expansion. See also: News and Views by Lerner & Petritsch Resource Top Nascent chromatin capture proteomics determines chromatin dynamics during DNA replication and identifies unknown fork components   pp281 - 293 Constance Alabert, Jimi-Carlo Bukowski-Wills, Sung-Bau Lee, Georg Kustatscher, Kyosuke Nakamura et al. doi:10.1038/ncb2918 Groth and colleagues identify proteins associated with newly synthesized DNA — isolated by nascent chromatin capture — as well as proteins associated with mature DNA, and find factors not previously linked to replication or nascent chromatin. 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