| PROTOCOLS | Top |
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Focal embolic cerebral ischemia in the rat pp539 - 547
In this model, the middle cerebral artery (MCA) is occluded with a fibrin-rich allogeneic clot, producing an embolic model of MCA occlusion in the rat that mimics the key components of neurovascular damage observed in human ischemic stroke.
Li Zhang et al.
Published online: 05 March 2015 | doi:10.1038/nprot.2015.036
Abstract | Full Text | PDF (1,983K)
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Hydrogenation using iron oxide–based nanocatalysts for the synthesis of amines pp548 - 557
Hydrogenation of functionalized substrates often relies on expensive noble metal–based catalysts. Jagadeesh et al. detail the hydrogenation of nitroarenes and the reductive amination of aldehydes with nitroarenes performed with an iron-based catalyst.
Rajenahally V Jagadeesh et al.
Published online: 05 March 2015 | doi:10.1038/nprot.2015.025
Abstract | Full Text | PDF (2,751K)
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Preparation of chiral quantum dots pp558 - 573
Optically active, chiral quantum dots can be prepared using chiral ligands. These nanoparticles have potential applications in photocatalysis and biological imaging, as well as in assays and sensors in asymmetric synthesis and enantioseparation.
Mícheál P Moloney et al.
Published online: 05 March 2015 | doi:10.1038/nprot.2015.028
Abstract | Full Text | PDF (2,189K)
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Live imaging of axonal transport in Drosophila pupal brain explants pp574 - 584
This protocol describes live imaging of axonal transport in Drosophila pupal brains. It complements previous techniques for imaging larval neurons by enabling the study of extensive changes occurring during metamorphosis.
Caroline Medioni, Anne Ephrussi and Florence Besse
Published online: 12 March 2015 | doi:10.1038/nprot.2015.034
Abstract | Full Text | PDF (2,679K)
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A two-component 'double-click' approach to peptide stapling pp585 - 594
This protocol describes an efficient double-click reaction between dialkynyl linkers and diazido peptides for generating peptides that are stabilized in an α-helical conformation. The method enables modular control over the staple linkage itself.
Yu Heng Lau et al.
Published online: 12 March 2015 | doi:10.1038/nprot.2015.033
Abstract | Full Text | PDF (1,162K)
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Preparation of abiotic polymer nanoparticles for sequestration and neutralization of a target peptide toxin pp595 - 604
Through screening libraries of polymer nanoparticles (NPs) containing various comonomers, it is possible to develop NPs with high affinity for target biomacromolecules. This protocol describes the preparation and characterization of melittin-binding NPs.
Keiichi Yoshimatsu, Hiroyuki Koide, Yu Hoshino and Kenneth J Shea
Published online: 19 March 2015 | doi:10.1038/nprot.2015.032
Abstract | Full Text | PDF (980K)
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Automated Cell Sorting - Now Available at a Bench near You
Take control of the how, when, and where of cell sorting with the S3e™, a benchtop instrument that makes cell sorting accessible to all. Automated setup and real-time sort monitoring eliminate the need for flow cytometry expertise, while a small footprint ensures that the S3e fits in almost any lab. Learn more. |  | |
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Simultaneous measurement of genome-wide transcription elongation speeds and rates of RNA polymerase II transition into active elongation with 4sUDRB-seq pp605 - 618
4sUDRB-seq is a genome-wide method for measuring the transcription rate of actively transcribed genes. It can provide information on the speed of elongation and on the rate at which RNA polymerase II makes the transition into active elongation.
Gilad Fuchs et al.
Published online: 26 March 2015 | doi:10.1038/nprot.2015.035
Abstract | Full Text | PDF (1,188K)
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Multiplexed locus-specific analysis of DNA methylation in single cells pp619 - 631
Cheow et al. provide a protocol for SCRAM, a method for determining DNA methylation status at defined target sites in single cells. It is reliable, low in cost and relatively fast, making it a good option when full genome coverage is not required.
Lih Feng Cheow, Stephen R Quake, William F Burkholder and Daniel M Messerschmidt
Published online: 26 March 2015 | doi:10.1038/nprot.2015.041
Abstract | Full Text | PDF (1,146K)
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Quantitative prediction of charge mobilities of π-stacked systems by first-principles simulation pp632 - 642
Carrier mobility is the most important parameter for assessing charge carrier transfer. This protocol describes a tool for predicting the charge carrier mobilities of π-stacked systems such as organic semiconductors and the DNA double helix.
Wei-Qiao Deng et al.
Published online: 26 March 2015 | doi:10.1038/nprot.2015.038
Abstract | Full Text | PDF (985K) |
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| CORRIGENDA | Top |
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Corrigendum: A standardized protocol for repeated social defeat stress in mice p644
Sam A Golden, Herbert E Covington, III, Olivier Berton and Scott J Russo
Published online: 26 March 2015 | doi:10.1038/nprot0415-644a
Full Text | PDF (538K)
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Corrigendum: Solid-phase proximity ligation assays for individual or parallel protein analyses with readout via real-time PCR or sequencing p644
Rachel Yuan Nong et al.
Published online: 26 March 2015 | doi:10.1038/nprot0415-644b
Full Text | PDF (538K)
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Corrigendum: Using TRIP for genome-wide position effect analysis in cultured cells p644
Waseem Akhtar et al.
Published online: 26 March 2015 | doi:10.1038/nprot0415-644c
Full Text | PDF (538K)
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Corrigendum: Functional genomics platform for pooled screening and generation of mammalian genetic interaction maps p644
Martin Kampmann, Michael C Bassik and Jonathan S Weissman
Published online: 26 March 2015 | doi:10.1038/nprot0415-644d
Full Text | PDF (538K) |
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| ERRATUM | Top |
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Erratum: Antibody structural modeling with prediction of immunoglobulin structure (PIGS) p644
Paolo Marcatili, Pier Paolo Olimpieri, Anna Chailyan and Anna Tramontano
Published online: 26 March 2015 | doi:10.1038/nprot0415-644e
Full Text | PDF (538K) |
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