Nature Biotechnology Contents: Volume 34 pp 785 - 890

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Advertisement A*STAR Research - Highlighting the best of research at the Agency for Science, Technology and Research (A*STAR), Singapore's premier research organization    Recent Highlights Bioimaging: Bringing brain tumors into focus | Biotechnology: A path away from reliance on oil | Cell biology: Moving DNA   Download the A*STAR Research app now!  TABLE OF CONTENTS August 2016 Volume 34, Issue 8 Editorial News Bioentrepreneur Opinion and Comment Features News and Views Research Careers and Recruitment Advertisement Nature Outlook Open Innovation In the competitive world of drug discovery and development, secrecy is no longer as important as it was. As it has become more difficult and costly to produce therapies, competitors have begun to view greater collaboration and openness as a way to improve the efficiency of research.  Available free online  Produced with support from  Boehringer Ingelheim Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement BIOPHARMA DEALMAKERS Company Profiles and Partnering Opportunities   Advertisement nature.com webcasts Nature Publishing Group presents a custom webcast on: Holistic workflow solutions to target, purify and characterize glycoprotein profiles Date: Thursday August 25, 2016 Time: 8AM PDT, 11AM EDT, 4PM BST, 5PM CEST  Register for the webcast and live Q&A session: Sponsored by: Thermo Fisher     Advertisement Microsystems & Nanoengineering is a fully open access, peer-reviewed journal, which publishes original articles and reviews on cutting-edge and emerging topics related to MEMS/ NEMS and nanotechnology. Submit your manuscript.   Editorial Top What Brexit means for biotech   p785 doi:10.1038/nbt.3657 Following the bombshell UK vote to leave the EU, Nature Biotechnology offers some cheer to the UK's former life sciences chief, George Freeman. News Top 'Brexit' stuns UK biotech into waiting game, but not all signals are red   pp787 - 788 Peter Mitchell doi:10.1038/nbt0816-787 Genentech's PD-L1 agent approved for bladder cancer   pp789 - 790 Mark Ratner doi:10.1038/nbt0816-789 Biogen and UPenn join forces to commercialize gene therapies   pp791 - 793 Cormac Sheridan doi:10.1038/nbt0816-791 Juno's wild ride   p793 Laura DeFrancesco doi:10.1038/nbt0816-793 Cancer anabolic metabolism inhibitors move into clinic   pp794 - 795 Ken Garber doi:10.1038/nbt0816-794 First-in-human CRISPR trial   p796 doi:10.1038/nbt0816-796a Synthetic human genome set to spur applications   pp796 - 797 Amber Dance doi:10.1038/nbt0816-796b Gilead's first pan-genotypic HCV cure   p797 doi:10.1038/nbt0816-797 Around the world in a month   p798 doi:10.1038/nbt0816-798 Data Page Drug pipeline: 2Q16   p799 Laura DeFrancesco doi:10.1038/nbt.3651 2Q16—biotech claws back   p800 Steve Edelson and Meredith Durkin Wolfe doi:10.1038/nbt.3654 Advertisement Hey there lab rat! 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Restrictions may apply, offer expires 10/31/16.  Bioentrepreneur Top Data page Research biotech patenting 2015   pp801 - 802 Brady Huggett and Kathryn Paisner doi:10.1038/nbt.3646 Podcast First Rounders Podcast: David Baltimore   p802 doi:10.1038/nbt.3642 Opinion and Comment Top Correspondence A crowdsourcing approach for reusing and meta-analyzing gene expression data OPEN   pp803 - 806 Naisha Shah, Yongjian Guo, Katherine V Wendelsdorf, Yong Lu, Rachel Sparks et al. doi:10.1038/nbt.3603 Targeted mutagenesis in mice by electroporation of Cpf1 ribonucleoproteins   pp807 - 808 Junho K Hur, Kyoungmi Kim, Kyung Wook Been, Gayoung Baek, Sunghyeok Ye et al. doi:10.1038/nbt.3596 Generation of knockout mice by Cpf1-mediated gene targeting   pp808 - 810 Yongsub Kim, Seung-A Cheong, Jong Geol Lee, Sang-Wook Lee, Myeong Sup Lee et al. doi:10.1038/nbt.3614 Nanopore development at Oxford Nanopore   pp810 - 811 Clive G Brown and James Clarke doi:10.1038/nbt.3622 Rapid, semi-automated protein terminal characterization using ISDetect   pp811 - 813 Corey E Bakalarski, Yutian Gan, Ingrid Wertz, Jennie R Lill and Wendy Sandoval doi:10.1038/nbt.3621 Features Top Patents The rise and fall of innovation in biofuels   pp814 - 821 Stevan C Albers, Annabelle M Berklund and Gregory D Graff doi:10.1038/nbt.3644 A look at the global landscape of biofuel patenting shows that, after surging between 2004 and 2008, the invention of biofuel technologies slowed considerably, and in many countries went into decline. Global trends point to an uncertain future, in particular, for advanced biofuels. Recent patents in synthetic biology   p822 doi:10.1038/nbt.3656 Advertisement   Therapeutic approaches to enhance natural killer cell cytotoxicity: the force awakens This Poster discusses the growing armamentarium of Natural Killer cell based anticancer approaches and provides an overview of the strategies and agents in preclinical and clinical research.  Download the Poster free online  Produced with support from Lonza Bioscience Solutions    News and Views Top Deep phenotyping predicts Huntington's genotype   pp823 - 824 Douglas M Ruderfer and Joel T Dudley doi:10.1038/nbt.3648 Comprehensive phenotyping of Huntington's disease in mice yields phenotypic signatures of disease-causing genetic variants. See also: Research by Alexandrov et al. An edible switch for gene therapy   pp824 - 825 Xavier M Anguela and Katherine A High doi:10.1038/nbt.3645 The expression of therapeutic transgenes in mice is made responsive to the amino acid content of the diet. Automated design of DNA origami   pp826 - 827 Veikko Linko and Mauri A Kostiainen doi:10.1038/nbt.3647 A top-down computational method enables fabrication of DNA objects in any shape. Research Highlights   p827 doi:10.1038/nbt.3649 Biotechnology JOBS of the week Senior Biochemist BluSense Diagnostics ApS Group Leader Cell Culture Research / Senior Scientist (m / f) Roche Diagnostics GmbH Exciting PhD Positions in Modern Biology VBC PhD Programme Director / Research Professor University of Antwerp - VIB More Science jobs from Biotechnology EVENT ICBEM 2016 6th International Conference on Biotechnology and Environmental Management September 24, 2016 Toronto, Canada More science events from Research Top Perspective Sharing and community curation of mass spectrometry data with Global Natural Products Social Molecular Networking   pp828 - 837 Mingxun Wang, Jeremy J Carver, Vanessa V Phelan, Laura M Sanchez, Neha Garg et al. doi:10.1038/nbt.3597 GNPS is an open-access community-curated analysis platform for sharing natural product mass spectrometry data that enables continuous, automatic reanalysis of deposited 'living' data sets. Articles Large-scale phenome analysis defines a behavioral signature for Huntington's disease genotype in mice   pp838 - 844 Vadim Alexandrov, Dani Brunner, Liliana B Menalled, Andrea Kudwa, Judy Watson-Johnson et al. doi:10.1038/nbt.3587 Analyzing and integrating a broad range of behavioral phenotypes enables prediction of underlying genetic mutations in mouse models of Huntington's disease. See also: News and Views by Ruderfer & Dudley A recellularized human colon model identifies cancer driver genes   pp845 - 851 Huanhuan Joyce Chen, Zhubo Wei, Jian Sun, Asmita Bhattacharya, David J Savage et al. doi:10.1038/nbt.3586 An ex vivo model of human colon cancer enables study of the drivers of tumor development and invasion. Letters Single-cell 5hmC sequencing reveals chromosome-wide cell-to-cell variability and enables lineage reconstruction   pp852 - 856 Dylan Mooijman, Siddharth S Dey, Jean-Charles Boisset, Nicola Crosetto and Alexander van Oudenaarden doi:10.1038/nbt.3598 A genome-wide, strand-specific sequencing method to detect 5-hydroxymethylcytosine marks in single cells is developed. Wide field-of-view, multi-region, two-photon imaging of neuronal activity in the mammalian brain   pp857 - 862 Jeffrey N Stirman, Ikuko T Smith, Michael W Kudenov and Spencer L Smith doi:10.1038/nbt.3594 Simultaneous imaging of neural activity in large regions of the mouse brain at subcellular resolution is made possible with a wide field-of-view two-photon microscope. Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells   pp863 - 868 Daesik Kim, Jungeun Kim, Junho K Hur, Kyung Wook Been, Sun-heui Yoon et al. doi:10.1038/nbt.3609 On-target and off-target specificities of Cfp1 are assessed and compared with SpCas9 in human cells. Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells   pp869 - 874 Benjamin P Kleinstiver, Shengdar Q Tsai, Michelle S Prew, Nhu T Nguyen, Moira M Welch et al. doi:10.1038/nbt.3620 On-target activities and genome-wide specificities of Cpf1 nucleases in human cells. Translation efficiency of mRNAs is increased by antisense oligonucleotides targeting upstream open reading frames   pp875 - 880 Xue-hai Liang, Wen Shen, Hong Sun, Michael T Migawa, Timothy A Vickers et al. doi:10.1038/nbt.3589 Antisense technology is used to increase the expression of specific therapeutic proteins in cells and in mice. Canola engineered with a microalgal polyketide synthase-like system produces oil enriched in docosahexaenoic acid   pp881 - 887 Terence A Walsh, Scott A Bevan, Daniel J Gachotte, Cory M Larsen, William A Moskal et al. doi:10.1038/nbt.3585 Canola oil containing healthful long chain omega-3 polyunsaturated fatty acids is produced from plants engineered with an algal polyketide synthase-like enzyme system. Errata Erratum: Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells   p888 Daesik Kim, Jungeun Kim, Junho K Hur, Kyung Wook Been, Sun-heui Yoon et al. doi:10.1038/nbt0816-888a Erratum: No longer going to waste   p888 Ken Garber doi:10.1038/nbt0816-888b Erratum: Nature Biotechnology's academic spinouts of 2015   p888 Aaron Bouchie and Laura DeFrancesco doi:10.1038/nbt0816-888c Erratum: Near-optimal probabilistic RNA-seq quantification   p888 Nicolas L Bray, Harold Pimentel, Pall Melsted and Lior Pachter doi:10.1038/nbt0816-888d Careers and Recruitment Top Second-quarter biotech job picture   p889 Michael Francisco doi:10.1038/nbt.3650 People People   p890 doi:10.1038/nbt.3655 Advertisement Nature Medicine and the Volkswagen Foundation present:  GENOME EDITING FOR GENE AND CELL THERAPY, A HERRENHAUSEN SYMPOSIUM November 3-4, 2016 | Herrenhausen Palace Conference Center, Hanover, Germany REGISTER NOW!   Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. 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